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Contrary to oligo(dT) primers, these primers are usually not template distinct and therefore are ideal for DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.
In contrast to random primers, oligo (dT) primers usually are not ideal for degraded samples but are great for setting up cDNA libraries and advisable for use with reverse transcriptases (RTs) as Superscript IV RT.
Retail outlet primers within their first packaging until finally able to use and handle Every primer with treatment to stop dropping or hanging them against challenging surfaces. Usually Keep to the company's guidelines and local security rules. five. How really should I shop my primers to keep up their good quality and longevity?
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